FASTQ format files are produced by nearly all modern sequencers and serve as a starting point for the bioinformatic analysis of next-generation sequencing (NGS) data. Understanding this format is key ...

A FASTQ file is a text file that stores the sequence data from clusters that pass the flow cell's filter. Demultiplexing is the first phase in creating a FASTQ file if specimens were multiplexed.

The term ‘RNA-seq’ refers to a collection of assays based on sequencing experiments that involve quantifying RNA species from bulk tissue, single cells or single nuclei. The kallisto, bustools and ...

Efficient data compression technologies are crucial to reduce the cost of long-term storage and file transfer in whole genome sequencing studies. This study benchmarked four specialized compression ...